Tre recombinase
In today's world, Tre recombinase is a topic that has captured the attention of people of all ages and cultures. Its relevance has been reflected in the extensive media coverage it has received, as well as in the growing interest it has aroused in different sectors of society. Experts and hobbyists alike have found reasons to dedicate time and resources to exploring this topic and its implications. In this article, we will examine Tre recombinase from different angles, analyzing its impact in various areas and offering perspectives to better understand its importance in the current context.
Tre recombinase is an experimental enzyme that in lab tests has removed DNA inserted by HIV from infected cells.[1] Through selective mutation, Cre recombinase which recognizes loxP sites are modified to identify HIV long terminal repeats (loxLTR) instead. As a result, instead of performing Cre-Lox recombination, the new enzyme performs recombination at HIV provirus sites.[2]
The structure of Tre in complex with loxLTR has been resolved (PDB: 5U91), allowing for analyzing the roles of individual mutations.[3]
References
- ^ Sarkar, Indrani; Hauber, Ilona; Hauber, Joachim; Buchholz, Frank (2007). "HIV-1 proviral DNA excision using an evolved recombinase". Science. 316 (5833): 1912–15. Bibcode:2007Sci...316.1912S. doi:10.1126/science.1141453. PMID 17600219. S2CID 2437602.
- ^ Hauber, Ilona; Hofmann-Sieber, Helga; Chemnitz, Jan; et al. (September 26, 2013). "Highly Significant Antiviral Activity of HIV-1 LTR-Specific Tre-Recombinase in Humanized Mice". PLOS Pathogens. 9 (9) e1003587. doi:10.1371/journal.ppat.1003587. PMC 3784474. PMID 24086129.
- ^ Meinke, G; Karpinski, J; Buchholz, F; Bohm, A (19 September 2017). "Crystal structure of an engineered, HIV-specific recombinase for removal of integrated proviral DNA". Nucleic Acids Research. 45 (16): 9726–9740. doi:10.1093/nar/gkx603. PMC 5766204. PMID 28934476.
External links
- Minkel, JR (June 28, 2007). "Designer enzyme cuts HIV out of infected cells". Scientific American.
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